Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mecp2

Cell type

Cell type Class
Neural
Cell type
Frontal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Frontal cortex
strain
129/FVB
genotype/variation
Cam-tTA:Fl-H1.0
chip antibody
MeCP2 (Millipore, #17-10491)
tissue
Frontal cortex

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and MeCP2-DNA complexes were isolated with Anti-MeCP2 antibody. Library was prepared by Genomic and RNA Profiling Core at Baylor College of Medicine using ThruPLEX DNA-seq kit (Rubicon).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
120282120
Reads aligned (%)
97.1
Duplicates removed (%)
17.4
Number of peaks
844 (qval < 1E-05)

mm9

Number of total reads
120282120
Reads aligned (%)
97.0
Duplicates removed (%)
17.5
Number of peaks
865 (qval < 1E-05)

Base call quality data from DBCLS SRA